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Determination of glufosfamide in rat plasma by liquid chromatography/tandem mass spectrometry
Author(s) -
Chen Xiaoyan,
Sun Yuming,
Cao Xueqin,
Jin Fengdan,
Zhong Dafang
Publication year - 2005
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1991
Subject(s) - chemistry , chromatography , protein precipitation , tandem mass spectrometry , electrospray ionization , mass spectrometry , liquid chromatography–mass spectrometry , electrospray , plasma , methanol , tandem , analytical chemistry (journal) , physics , materials science , organic chemistry , quantum mechanics , composite material
A sensitive and selective high‐performance analytical method based on liquid chromatography with tandem mass spectrometric detection (LC/MS/MS) was developed for the quantification of glufosfamide in rat plasma. Zidovudine was employed as internal standard. Glufosfamide was determined after methanol‐mediated plasma protein precipitation using LC/MS/MS with an electrospray ionization interface in negative ion mode. Two sets of standard curves were developed, from 0.005 to 1.0 µg/mL and from 1.0 to 50.0 µg/mL. The assay was accurate (% deviations from nominal concentrations < 5%), precise and reproducible (intra‐ and inter‐day coefficients of variation < 10%). Glufosfamide in rat plasma was stable over three freeze/thaw cycles, and at ambient temperatures, for at least 2 h. The validated method was successfully applied to the determination of glufosfamide plasma concentrations in rats for 24 h following an intravenous administration of 25 mg/kg. Copyright © 2005 John Wiley & Sons, Ltd.