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Rapid screening of drugs of abuse and their metabolites by gas chromatography/mass spectrometry: application to urinalysis
Author(s) -
StranoRossi Sabina,
Molaioni Francesco,
Rossi Francesca,
Botrè Francesco
Publication year - 2005
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1942
Subject(s) - chemistry , urinalysis , chromatography , drugs of abuse , mass spectrometry , gas chromatography , gas chromatography–mass spectrometry , urine , pharmacology , drug , medicine , biochemistry
This paper describes a rapid gas chromatographic/mass spectrometric (GC/MS) screening method for the detection of drugs of abuse and/or their metabolites in urine. Synthetic stimulants, opiates, cocaine metabolites, cannabinoids—and specifically the acid metabolite of tetrahydrocannabinol (THC‐COOH)—can be simultaneously extracted by a single liquid/liquid separation step, at alkaline pH, and assayed as trimethylsilyl derivatives by GC/MS in SIM (selected ion monitoring) mode. All the analytes show a good linearity (R 2  > 0.99 for most of the considered substances) in the range 25–1000 ng/mL, with a good reproducibility of both the retention times (CV% <0.7) and the relative abundances of the characteristic diagnostic ions (CV% <13). The limit of detection (LOD) of the method is 25 ng/mL of target compound in human urine for most of the substances investigated, 3 ng/mL for THC‐COOH, and 10 ng/mL for norbuprenorphine. Validation of the method allows its application to different fields of forensic analytical toxicology, including antidoping analysis. Copyright © 2005 John Wiley & Sons, Ltd.

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