Premium
Photodissociation tandem mass spectrometry at 266 nm of an aliphatic peptide derivatized with phenyl isothiocyanate and 4‐sulfophenyl isothiocyanate
Author(s) -
Oh Joo Yeon,
Moon Jeong Hee,
Lee Yong Ho,
Hyung SeokWon,
Lee SangWon,
Kim Myung Soo
Publication year - 2005
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1922
Subject(s) - chemistry , derivatization , isothiocyanate , photodissociation , tandem mass spectrometry , tandem , mass spectrometry , protonation , mass spectrum , photochemistry , chromophore , molecule , peptide , chromatography , ion , analytical chemistry (journal) , organic chemistry , biochemistry , materials science , composite material
Photodissociation (PD) tandem mass spectra have been obtained at 266 nm for the protonated molecules of a tryptic peptide, ASHLGLAR, and of its phenyl isothiocyanate and 4‐sulfophenyl isothiocyanate derivatives, generated by matrix‐assisted laser desorption/ionization. Derivatization with the aromatic chromophores greatly reduced the intensity of the laser required for efficient PD. Major fragment ions observed in the three spectra are quite similar. General features of the PD tandem mass spectra and their potential utility for analytical purposes are discussed. Copyright © 2005 John Wiley & Sons, Ltd.