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The detection of modafinil and its major metabolite in equine urine by liquid chromatography/mass spectrometry
Author(s) -
McKinney Andrew R.,
Suann Craig J.,
Stenhouse Allen M.
Publication year - 2005
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1910
Subject(s) - modafinil , chemistry , chromatography , metabolite , urine , mass spectrometry , solid phase extraction , liquid chromatography–mass spectrometry , extraction (chemistry) , pharmacology , biochemistry , medicine
Abstract A method has been developed for the detection of modafinil and its major metabolite, modafinil acid, in equine urine by solid‐phase extraction and positive ion electrospray ionisation liquid chromatography/mass spectrometry. The method has been applied to the analysis of equine urine samples obtained after the oral administration of modafinil. Modafinil acid was the major component in the urine, and was detected up to 4 days post‐administration. Unchanged modafinil was present at substantially lower concentrations, and was detected for only 24 hours. Copyright © 2005 John Wiley & Sons, Ltd.