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Improved matrix‐assisted laser desorption/ionisation sample preparation of a partially depolymerised cellulose derivative by continuous spray deposition and interfacing with size‐exclusion chromatography
Author(s) -
Momcilovic Dane,
Wahlund KarlGustav,
Wittgren Bengt,
Brinkmalm Gunnar
Publication year - 2005
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1874
Subject(s) - chemistry , chromatography , cellulose , analytical chemistry (journal) , analyte , sample preparation , mass spectrometry , aqueous solution , desorption , matrix (chemical analysis) , size exclusion chromatography , reproducibility , organic chemistry , adsorption , enzyme
Abstract Continuous spray deposition (CSD) of aqueous solutions of partially depolymerised methyl cellulose was found to improve matrix‐assisted laser desorption/ionisation (MALDI) sample preparation. One feature was that the sensitivity in MALDI time‐of‐flight mass spectrometry increased up to an order of magnitude compared with the standard sample preparation method. Another feature was that CSD provided targets for MALDI with homogeneously distributed analyte. This resulted in a more even signal intensity and a higher reproducibility than in the standard method. High‐mass discrimination was more pronounced in CSD than in the standard method. Size‐exclusion chromatography with aqueous eluent was coupled online to CSD onto matrix‐precoated foils. The suitability for determination of the molar mass distribution of methyl cellulose was investigated. Copyright © 2005 John Wiley & Sons, Ltd.