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Effect of post‐excitation radius on ion abundance, mass measurement accuracy, and isotopic distributions in Fourier transform ion cyclotron resonance mass spectrometry
Author(s) -
Hawkridge Adam M.,
Nepomuceno Angelito I.,
Lovik Stephanie L.,
Mason Christopher J.,
Muddiman David C.
Publication year - 2005
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1871
Subject(s) - fourier transform ion cyclotron resonance , chemistry , natural abundance , mass spectrometry , ion , ion cyclotron resonance , selected ion monitoring , analytical chemistry (journal) , excitation , radius , isotope , calibration , atomic physics , resonance (particle physics) , fourier transform , cyclotron , physics , nuclear physics , chromatography , gas chromatography–mass spectrometry , computer security , organic chemistry , quantum mechanics , computer science
We report an evaluation of a modern Fourier transform ion cyclotron resonance mass spectrometry (FT‐ICR‐MS) instrument to determine the general trend of post‐excitation radius on total ion abundance, mass measurement accuracy, and isotopic distributions for internally calibrated mass spectra. The optimum post‐excitation radius was determined using total ion abundance, mass measurement accuracy (MMA), and isotope ratios. However, despite the utility of internal calibration for achieving ultimate MMA, the internal calibrant ions were insufficient for compensating for sub‐optimum ICR cell conditions. The findings presented herein underscore the importance of determining the optimal post‐excitation radius in FT‐ICR‐MS to achieve high ion abundance (low limits of detection), high MMA, and valid isotopic distributions. Copyright © 2005 John Wiley & Sons, Ltd.