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Design and performance of a sheathless capillary electrophoresis/mass spectrometry interface by combining fused‐silica capillaries with gold‐coated nanoelectrospray tips
Author(s) -
Kele Zoltán,
Ferenc Györgyi,
Klement Éva,
Tóth Gábor K.,
Janáky Tamás
Publication year - 2005
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1866
Subject(s) - chemistry , capillary electrophoresis , capillary action , capillary electrophoresis–mass spectrometry , chromatography , coating , mass spectrometry , capillary electrochromatography , analytical chemistry (journal) , electrospray ionization , composite material , materials science , organic chemistry
A simple sheathless capillary electrophoresis (CE)/mass spectrometry (MS) interface was constructed by combining widely used nanospray needles with fused‐silica capillaries and it was successfully applied for the separation of peptides. The end of the CE capillary was pulled to a taper, etched and then fitted into the metal‐coated nanospray borosilicate capillary. The nanospray needle can be used for several CE runs, but it can be easily and rapidly changed in the case of accidental breakage or evaporation of the coating. A fast capillary electrochromatographic method was also developed for MS analysis of peptides containing numerous basic amino acids. Copyright © 2005 John Wiley & Sons, Ltd.