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A tandem mass spectrometric study of saccharides at high mass resolution
Author(s) -
March Raymond E.,
Stadey Christopher J.
Publication year - 2005
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1860
Subject(s) - chemistry , lactose , mass spectrum , monosaccharide , tandem mass spectrometry , electrospray ionization , mass spectrometry , glycosidic bond , protonation , chromatography , analytical chemistry (journal) , ion , organic chemistry , enzyme
Nine monosaccharides and four disaccharides were mass analyzed using a quadrupole time‐of‐flight tandem mass spectrometer combined with an electrospray ionization source. Product ion mass spectra of deprotonated, protonated, and sodiated saccharides were observed and were compared within each group of saccharides. Each of the deprotonated pentoses, hexoses and disaccharides yielded a significantly different product ion mass spectrum with the exception of α ‐lactose and β ‐lactose. The disaccharides α ‐ and β ‐lactose differ only at the glycosidic linkage. Product ion mass spectra of protonated and sodiated α ‐ and β ‐lactose were indistinguishable also. Copyright © 2005 John Wiley & Sons, Ltd.