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The use of turbulent flow chromatography and the isocratic focusing effect to achieve on‐line cleanup and concentration of neat biological samples for low‐level metabolite analysis
Author(s) -
Herman J. L.
Publication year - 2005
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1843
Subject(s) - chemistry , chromatography , metabolite , elution , mass spectrometry , flow (mathematics) , sample (material) , sample preparation , column (typography) , analytical chemistry (journal) , biochemistry , geometry , mathematics , structural engineering , connection (principal bundle) , engineering
The use of turbulent flow chromatography in conjunction with column switching isocratic focusing was used to perform on‐line sample cleanup and concentration of neat rat plasma for the identification of low‐level metabolites. The concentration was achieved by focusing multiple injections, which were cleaned by a turbulent flow column, onto an analytical column prior to elution into the mass spectrometer. In addition, the first application of turbulent flow chromatography for on‐line sample cleanup of neat bile samples is reported. The on‐line cleanup and concentration method extracts and concentrates a sample 20‐fold in 1 h, and is completely automated. Copyright © 2005 John Wiley & Sons, Ltd.