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Rapid determination of acetone in human blood by derivatization with pentafluorobenzyl hydroxylamine followed by headspace liquid‐phase microextraction and gas chromatography/mass spectrometry
Author(s) -
Deng Chunhui,
Li Ning,
Wang Xiaochuan,
Zhang Xiangmin,
Zeng Jia
Publication year - 2005
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1834
Subject(s) - chemistry , derivatization , chromatography , acetone , detection limit , mass spectrometry , gas chromatography–mass spectrometry , extraction (chemistry) , gas chromatography , solvent , organic chemistry
In the current work, a simple, rapid, accurate and inexpensive method was developed for the determination of acetone in human blood. The proposed method is based on derivatization with O ‐(2,3,4,5,6‐pentafluorobenzyl)hydroxylamine hydrochloride (PFBHA), followed by headspace liquid‐phase microextraction (HS‐LPME) and gas chromatography/mass spectrometry (GC/MS). In the present method, acetone in blood samples was derivatized with PFBHA and acetone oxime formed in several seconds. The formed oxime was enriched by HS‐LPME using the organic solvent film (OSF) formed in a microsyringe barrel as extraction interface. Finally, the enriched oxime was analyzed by GC/MS in electron ionization (EI) mode. HS‐LPME parameters including solvent, syringe plunger withdrawal rate, sampling volume, and extraction cycle were optimized and the method reproducibility, linearity, recovery and detection limit were studied. The proposed method was applied to determination of acetone in diabetes blood and normal blood. It has been shown that derivatization with HS‐LPME and GC/MS is an alternative method for determination of the diabetes biomarker, acetone, in blood samples. Copyright © 2005 John Wiley & Sons, Ltd.