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Gold coating of non‐conductive membranes before matrix‐assisted laser desorption/ionization tandem mass spectrometric analysis prevents charging effect
Author(s) -
Scherl Alexander,
ZimmermannIvol Catherine G.,
Dio Joel Di,
Vaezzadeh Ali R.,
Binz PierreAlain,
AmezDroz Michel,
Cochard Roland,
Sanchez JeanCharles,
Glückmann Matthias,
Hochstrasser Denis F.
Publication year - 2005
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1831
Subject(s) - chemistry , tandem , tandem mass spectrometry , mass spectrometry , membrane , analytical chemistry (journal) , coating , desorption , ionization , matrix assisted laser desorption/ionization , protein mass spectrometry , chromatography , ion , adsorption , organic chemistry , biochemistry , materials science , composite material
Acquisition of tandem mass spectra from peptides or other analytes deposited on non‐conductive membranes is inhibited on instruments combining matrix‐assisted laser desorption/ionization with tandem time‐of‐flight analyzers (MALDI‐TOF/TOF™) due to a charging effect. A thin layer of gold renders the membrane conductive. This allows adequate data acquisition on MALDI‐TOF/TOF™ systems. Therefore, this methodology extends the capacity of the molecular scanner concept to tandem mass spectrometry. Copyright © 2005 John Wiley & Sons, Ltd.