Gold coating of non‐conductive membranes before matrix‐assisted laser desorption/ionization tandem mass spectrometric analysis prevents charging effect | Zendy

Scherl Alexander | Zendy; ZimmermannIvol Catherine G. | Zendy; Dio Joel Di | Zendy; Vaezzadeh Ali R. | Zendy; Binz PierreAlain | Zendy; AmezDroz Michel | Zendy; Cochard Roland | Zendy; Sanchez JeanCharles | Zendy; Glückmann Matthias | Zendy; Hochstrasser Denis F. | Zendy

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Gold coating of non‐conductive membranes before matrix‐assisted laser desorption/ionization tandem mass spectrometric analysis prevents charging effect

Author(s) -

Scherl Alexander,

ZimmermannIvol Catherine G.,

Dio Joel Di,

Vaezzadeh Ali R.,

Binz PierreAlain,

AmezDroz Michel,

Cochard Roland,

Sanchez JeanCharles,

Glückmann Matthias,

Hochstrasser Denis F.

Publication year - 2005

Publication title -

rapid communications in mass spectrometry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.528

H-Index - 136

eISSN - 1097-0231

pISSN - 0951-4198

DOI - 10.1002/rcm.1831

Subject(s) - chemistry , tandem , tandem mass spectrometry , mass spectrometry , membrane , analytical chemistry (journal) , coating , desorption , ionization , matrix assisted laser desorption/ionization , protein mass spectrometry , chromatography , ion , adsorption , organic chemistry , biochemistry , materials science , composite material

Acquisition of tandem mass spectra from peptides or other analytes deposited on non‐conductive membranes is inhibited on instruments combining matrix‐assisted laser desorption/ionization with tandem time‐of‐flight analyzers (MALDI‐TOF/TOF™) due to a charging effect. A thin layer of gold renders the membrane conductive. This allows adequate data acquisition on MALDI‐TOF/TOF™ systems. Therefore, this methodology extends the capacity of the molecular scanner concept to tandem mass spectrometry. Copyright © 2005 John Wiley & Sons, Ltd.

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