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Investigation of the metabolic fate of 2‐, 3‐ and 4‐bromobenzoic acids in bile‐duct‐cannulated rats by inductively coupled plasma mass spectrometry and high‐performance liquid chromatography/inductively coupled plasma mass spectrometry/electrospray mass spectrometry
Author(s) -
Jensen Berit Packert,
Smith Christopher J.,
Bailey Christopher J.,
Rodgers Colin,
Wilson Ian D.,
Nicholson Jeremy K.
Publication year - 2005
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1822
Subject(s) - chemistry , chromatography , glucuronide , metabolite , urine , mass spectrometry , inductively coupled plasma mass spectrometry , high performance liquid chromatography , metabolism , biochemistry
Inductively coupled plasma mass spectrometry (ICPMS) has been used to determine the rate and routes of excretion of bromine following the intraperitoneal administration (50 mg kg −1 ) of 2‐, 3‐ and 4‐bromobenzoic acids to male bile‐duct‐cannulated rats. Analysis of urine and bile for 79/81 Br using ICPMS showed that all three bromobenzoic acids were rapidly excreted (82–98%) within 48 h of dosing, primarily via the urine. High‐performance liquid chromatography/inductively coupled plasma mass spectrometry (HPLC/ICPMS) was then used to obtain metabolite profiles for bile and urine. These profiles revealed that extensive metabolism had taken place, with the unchanged bromobenzoic acids forming a minor part of the total of compound‐related material detected. Concomitant MS studies, supplemented by alkaline hydrolysis, enabled the identification of the major metabolite of all three of the bromobenzoic acids as a glycine conjugate. Ester glucuronide conjugates were also identified, but formed only a small proportion of total. Copyright © 2005 John Wiley & Sons, Ltd.