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Simultaneous determination of zidovudine and lamivudine from rat tissues by liquid chromatography/tandem mass spectrometry
Author(s) -
Alnouti Yazen,
Lewis Summer R.,
White Catherine A.,
Bartlett Michael G.
Publication year - 2005
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1816
Subject(s) - chemistry , chromatography , lamivudine , zidovudine , protein precipitation , liquid chromatography–mass spectrometry , tandem mass spectrometry , ammonium acetate , amniotic fluid , mass spectrometry , high performance liquid chromatography , hydrophilic interaction chromatography , fetus , human immunodeficiency virus (hiv) , pregnancy , virus , virology , hepatitis b virus , genetics , viral disease , biology
A simple liquid chromatography/tandem mass spectrometry (LC/MS/MS) method has been developed and validated for the simultaneous determination of zidovudine (AZT) and lamivudine (3TC) in rat plasma, amniotic fluid, placental, and fetal tissues. Samples were processed by acetonitrile precipitation. Chromatography was performed using a C 18 column (5 μm, 150 × 3.9 mm i.d). The mobile phase consisted of 30% methanol and 7.5 mM ammonium acetate (pH 6.5). The method was validated in the range of 0.05–25 μg/mL for both 3TC and AZT in the four biological matrices. Finally, the method was applied to a study involving fetal transport following co‐administration of these compounds at a dose of 25 mg/kg each in a pregnant rat. Copyright © 2005 John Wiley & Sons, Ltd.