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Ionization and fragmentation of N ‐linked glycans as silver adducts by electrospray mass spectrometry
Author(s) -
Harvey David J.
Publication year - 2005
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1815
Subject(s) - chemistry , glycosidic bond , adduct , electrospray , electrospray ionization , fragmentation (computing) , mass spectrometry , dissociation (chemistry) , collision induced dissociation , ion , glycan , chromatography , tandem mass spectrometry , organic chemistry , glycoprotein , biochemistry , enzyme , computer science , operating system
[M+Ag] + ions were produced by electrospray from neutral high‐mannose, hybrid and complex N ‐linked glycans obtained from bovine ribonuclease, chicken egg glycoproteins, bovine fetuin and porcine thyroglobulin by the addition of silver nitrate to the electrospray solvent. Both singly and doubly charged ions were produced but, as the signals were split between the two silver isotopes, sensitivity was not as high as with the sodium adducts reported earlier. Collision‐induced dissociation (CID) spectra were dominated by ions produced by glycosidic cleavages, mainly of the B‐ and Y‐type. Internal cleavage ions involving both B and Y cleavages were very prominent but cross‐ring fragments were generally of very low abundance or absent. Silver was very efficient at cleaving the glycosidic bonds, so much so that spectra tended to contain glycosidic ions at most possible combinations of the constituent monosaccharides. Copyright © 2005 John Wiley & Sons, Ltd.