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Benzene‐assisted atmospheric‐pressure chemical ionization: a new liquid chromatography/mass spectrometry approach to the analysis of selected hydrophobic compounds
Author(s) -
Perazzolli Chiara,
Mancini Ines,
Guella Graziano
Publication year - 2005
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1807
Subject(s) - chemistry , atmospheric pressure chemical ionization , chromatography , chemical ionization , mass spectrometry , benzene , biphenyl , analytical chemistry (journal) , ionization , organic chemistry , ion
Charge‐exchange reactions involving benzene have been successfully exploited to increase the sensitivity of atmospheric‐pressure chemical ionization mass spectrometry (APCI‐MS) towards hydrophobic compounds of significant environmental relevance which are not detectable with the ordinary APCI techniques. Among them, good sensitivity have been found for (a) highly chlorinated biphenyl derivatives such as dichlorodiphenyltrichloroethane (DDT), dichlorodiphenyldichloroethane (DDD) and dichlorodiphenyldichloroethene (DDE); (b) cyclopentadienes such as Aldrin and its epoxy derivatives Dieldrin and Endrin; and (c) dibenzofurans and dibenzo‐ para ‐dioxins such as 2,3,7,8‐tetrachlorodibenzofuran (2,3,7,8‐TCDF) and 2,3,7,8‐tetrachloro‐dibenzo‐ para ‐dioxin (2,3,7,8‐TCDD). The reactant benzene molecules were introduced into the source either through the nebulizer gas or by direct post‐column addition of neat liquid, whereas the targeted compounds were analyzed using a high‐performance liquid chromatography (HPLC) RP‐18 column using methanol/water solutions as mobile phase. By using benzene as post‐column reagent, positive ion mode detection was proven to be significantly enhanced as compared with APCI measurements carried out without benzene assistance. Copyright © 2005 John Wiley & Sons, Ltd.