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Development of a liquid chromatography/electrospray tandem mass spectrometry assay for the quantification of apicidin, a novel histone deacetylase inhibitor, in rat serum: application to a pharmacokinetic study
Author(s) -
Shin Beom Soo,
Kim John,
Yoon Chi Ho,
Kim Chul Hwan,
Park Eun Hye,
Han JeungWhan,
Yoo Sun Dong
Publication year - 2005
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1805
Subject(s) - chemistry , chromatography , pharmacokinetics , liquid chromatography–mass spectrometry , selected reaction monitoring , tandem mass spectrometry , metabolite , mass spectrometry , electrospray ionization , pharmacology , biochemistry , medicine
Abstract Apicidin, a fungal metabolite isolated from Fusarium pallidoroseum , is a cyclic tetrapeptide that exhibits potent anti‐protozoal and anti‐angiogenic activities. Although extensive studies have been recently conducted to examine the biological and pharmacological action, no information is available on the quantitative analysis of apicidin. To our knowledge, this study is the first to describe a rapid and sensitive liquid chromatography/tandem mass spectrometry (LC/MS/MS) assay method for the quantification of apicidin in rat serum. The method was validated to demonstrate the specificity, linearity, recovery, lower limit of quantification (LLOQ), accuracy, and precision. The multiple reaction monitoring was based on the transitions m/z 624.7 → 84.3 and 372.1 → 176.1 for apicidin and trazodone, respectively. The assay utilized a single liquid‐liquid extraction and isocratic elution, and the LLOQ was 0.5 ng/mL using 0.1 mL of rat serum. The assay was linear over a range from 0.5–1000 ng/mL, with correlation coefficients >0.9994. The mean intra‐ and inter‐day assay accuracy ranged from 99.9–101.5% and 94.8–102.1%, respectively, and the mean intra‐ and inter‐day precision was between 2.7–5.9% and 1.6–11.5%, respectively. The developed assay method was applied to a pharmacokinetic study after intravenous injection of apicidin in rats at a dose of 1 mg/kg. Copyright © 2005 John Wiley & Sons, Ltd.