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Determination of N 9 / N 7 ‐isomer ratio of alkyl (guaninyl)acetates by electrospray ionization tandem mass spectrometry
Author(s) -
Ferenc Györgyi,
Kele Zoltán,
Kovács Lajos
Publication year - 2004
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1774
Subject(s) - chemistry , electrospray ionization , structural isomer , tandem mass spectrometry , mass spectrometry , alkyl , alkylation , electrospray , tandem , ionization , analytical chemistry (journal) , medicinal chemistry , chromatography , ion , organic chemistry , catalysis , materials science , composite material
Abstract N 9 ‐ and N 7 ‐Substituted (guaninyl)acetic esters were studied by electrospray ionization tandem mass spectrometry (ESI‐MS/MS) in order to determine their ratio in alkylation reactions. The loss of ammonia is significantly different for the N 9 ‐ and N 7 ‐alkylated guanine regioisomer pairs. More importantly, the abundance of the [MH–17] + ion is in linear correlation with the N 9 ‐isomer content. Therefore, the ratio of regioisomers can be determined in a mixture containing these compounds. Copyright © 2004 John Wiley & Sons, Ltd.