Premium
Probing the interaction of kojic acid antibiotics with iron(III) chloride by using electrospray tandem mass spectrometry
Author(s) -
Sudhir PuttyReddy,
Wu HuiFen,
Zhou ZiCong
Publication year - 2004
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1773
Subject(s) - chemistry , kojic acid , electrospray ionization , tandem mass spectrometry , mass spectrometry , chloride , chelation , fragmentation (computing) , electrospray , chromatography , nuclear chemistry , inorganic chemistry , organic chemistry , tyrosinase , computer science , enzyme , operating system
Kojic acid, 5‐hydroxy‐2‐(hydroxymethyl)‐4 H ‐pyran‐4‐one, has been used extensively as a clinical iron‐chelating drug although the nature of the complexes of iron and kojic acid has not been established. In this article we demonstrate the complexation of kojic acid with iron(III) chloride by using electrospray ionization mass spectrometry (ESI‐MS). The ESI‐MS analysis revealed different reactions between iron(III) chloride and kojic acid (M), and the mass spectrum exhibited four complexes: [Fe+2(MH)] + , [Fe+3(MH)+H] + , [Fe 2 +4(MH)+Cl] + , and [Fe 2 +5(MH)] + . All these proposed complexes and the presence of chloride ion in one of the dinuclear complexes have been confirmed by isotopic patterns and fragmentation studies by means of tandem mass spectrometry (MS n ). Copyright © 2004 John Wiley & Sons, Ltd.