Premium
Atmospheric pressure chemical ionisation liquid chromatography/multistage mass spectrometry for assignment of sedimentary bacteriochlorophyll derivatives
Author(s) -
Wilson Michael A.,
Hodgson Dominic A.,
Keely Brendan J.
Publication year - 2004
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1749
Subject(s) - chemistry , mass spectrometry , chromatography , atmospheric pressure chemical ionization , mass spectrum , atmospheric pressure , isobaric process , sediment , ionization , analytical chemistry (journal) , chemical ionization , organic chemistry , ion , oceanography , geology , paleontology , physics , biology , thermodynamics
Atmospheric pressure chemical ionisation liquid chromatography/multistage mass spectrometry (APCI‐LC/MS n ) provides a rapid, on‐line method for the assignment of individual bacteriophaeophorbide c and d methyl esters (BPMEs) in complex mixtures. The MS 2 spectrum for each component is diagnostic of the type of BPME ( c or d ), and characteristic losses in MS 5 and MS 6 permit assignment of the alkyl substituents at positions C‐8 and C‐12 of the macrocycle. MS 5 mass chromatograms permit the deconvolution of coeluting isobaric BPMEs, revealing the true profiles of the individual components. The distributions are different in lake sediments from la Salada de Chiprana (Spain) and Kirisjes Pond (Antarctica), and a novel BPME c with a neo ‐pentyl substituent has been observed in the Kirisjes Pond sediment. Copyright © 2004 John Wiley & Sons, Ltd.