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Coupling of nanoflow liquid chromatography to matrix‐assisted laser desorption/ionization mass spectrometry: real‐time liquid chromatography run mapping on a MALDI plate
Author(s) -
Nägele Edgar,
Vollmer Martin
Publication year - 2004
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1723
Subject(s) - chemistry , chromatography , mass spectrometry , maldi imaging , direct electron ionization liquid chromatography–mass spectrometry interface , analytical chemistry (journal) , ionization , matrix (chemical analysis) , spotting , surface enhanced laser desorption/ionization , desorption , resolution (logic) , high performance liquid chromatography , capillary electrophoresis–mass spectrometry , sample preparation , sample preparation in mass spectrometry , matrix assisted laser desorption/ionization , electrospray ionization , chemical ionization , adsorption , optics , ion , physics , organic chemistry , artificial intelligence , computer science
The major obstacle in the use of matrix‐assisted laser desorption/ionization mass spectrometry (MALDI‐MS) instruments in the analysis of complex proteome samples is the lack of a direct coupling of a highly resolving separation technique with the mass spectrometer itself. To overcome this drawback, a spotting device for capillary and nanoflow liquid chromatography (LC) with a special liquid deposition principle for lowest volumes was developed. The instrument is able to perform MALDI spotting in real time in order to deposit the LC run on the MALDI plate, and therefore couples the high resolution power of nano‐RP‐HPLC separation directly with MALDI‐MS. This work describes the development and optimization of a method for spotting with online matrix addition, and illustrates its use in the analysis of a complex proteome sample. Copyright © 2004 John Wiley & Sons, Ltd.