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Capillary electrophoresis off‐line matrix‐assisted laser desorption/ionisation mass spectrometry of intact and digested proteins using cationic‐coated capillaries
Author(s) -
Zuberovic Aida,
Ullsten Sara,
Hellman Ulf,
Markides Karin E.,
Bergquist Jonas
Publication year - 2004
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1712
Subject(s) - chemistry , mass spectrometry , chromatography , capillary electrophoresis , desorption , capillary electrophoresis–mass spectrometry , matrix assisted laser desorption/ionization , cationic polymerization , protein mass spectrometry , adsorption , electrophoresis , matrix (chemical analysis) , analytical chemistry (journal) , capillary action , tandem mass spectrometry , electrospray ionization , polymer chemistry , organic chemistry , materials science , composite material
Capillary electrophoresis (CE) was coupled off‐line with matrix‐assisted laser desorption/ionisation time‐of‐flight mass spectrometry (MALDI‐TOFMS) for the analysis of proteins and peptides. CE fractions were collected directly on a matrix‐coated MALDI target, using a sheath‐flow interface. Protein adsorption during CE separations was prevented by coating the capillaries with the physically adsorbed, cationic polymer PolyE‐323. The CE/MALDI‐MS system was used for the analysis of model proteins and peptides at physiological pH as well as analysis of proteins in tear fluid. Moreover, tryptic on‐target digestion of the collected protein fractions, with subsequent MALDI‐MS and MS/MS peptide analysis, was demonstrated. Copyright © 2004 John Wiley & Sons, Ltd.