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Simultaneous determination of magnesium lithospermate B, rosmarinic acid, and lithospermic acid in beagle dog serum by liquid chromatography/tandem mass spectrometry
Author(s) -
Li Xiaochuan,
Yu Chen,
Sun Weikang,
Liu Gangyi,
Jia Jingying,
Wang Yiping
Publication year - 2004
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1703
Subject(s) - chemistry , beagle , rosmarinic acid , chromatography , pharmacokinetics , liquid chromatography–mass spectrometry , quantitative analysis (chemistry) , tandem mass spectrometry , high performance liquid chromatography , mass spectrometry , extraction (chemistry) , pharmacology , biochemistry , medicine , antioxidant
A rapid, sensitive and specific isocratic liquid chromatography/tandem mass spectrometry (LC/MS/MS) method was developed for the simultaneous quantitative determination of magnesium lithospermate B (MLB), rosmarinic acid (RA), and lithospermic acid (LA) in beagle dog serum, with silibinin as internal standard. The serum samples were treated by liquid‐liquid extraction and analyzed using LC/MS/MS with a TurboIonSpray source. A short run‐time (3 min) fulfilled the need for monitoring serum levels of MLB, RA, and LA in large‐scale studies. The calibration curves for MLB, RA, and LA were linear over the ranges 8–2048, 4–1024, and 4–1024 ng/mL, respectively, with coefficients of correlation >0.999. The intra‐ and inter‐day precision (CV) of analysis was <10%, and accuracy ranged from 90–104%. This quantitation method was successfully applied to a pharmacokinetic study of salvianolate administrated by intravenous infusion with dosage of 6 mg/kg in beagle dogs. Copyright © 2004 John Wiley & Sons, Ltd.