Analysis of urinary nucleosides. IV. Identification of urinary purine nucleosides by liquid chromatography/electrospray mass spectrometry | Zendy

Dudley Edward | Zendy; Lemière Filip | Zendy; Van Dongen Walter | Zendy; Tuytten Robin | Zendy; ElSharkawi Salah | Zendy; Brenton A. Gareth | Zendy; Esmans Eddy L. | Zendy; Newton Russell P. | Zendy

Premium

Analysis of urinary nucleosides. IV. Identification of urinary purine nucleosides by liquid chromatography/electrospray mass spectrometry

Author(s) -

Dudley Edward,

Lemière Filip,

Van Dongen Walter,

Tuytten Robin,

ElSharkawi Salah,

Brenton A. Gareth,

Esmans Eddy L.,

Newton Russell P.

Publication year - 2004

Publication title -

rapid communications in mass spectrometry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.528

H-Index - 136

eISSN - 1097-0231

pISSN - 0951-4198

DOI - 10.1002/rcm.1685

Subject(s) - chemistry , chromatography , mass spectrometry , inosine , urine , electrospray mass spectrometry , high performance liquid chromatography , purine , tandem mass spectrometry , liquid chromatography–mass spectrometry , urinary system , purine metabolism , electrospray , adenosine , biochemistry , enzyme , medicine , endocrinology

Modified urinary nucleosides are potentially invaluable in cancer diagnosis. High‐performance liquid chromatography (HPLC) was combined with full scan mass spectrometry (MS), tandem mass spectrometry and MS n analysis in order to identify purine nucleosides purified from urine. UV peaks evident in the chromatogram were examined by the various mass spectrometric techniques and adenosine, 1‐methyladenosine, xanthosine, N 1 ‐methylguanosine, N 2 ‐methylguanosine, N 2 , N 2 ‐dimethylguanosine, N 2 , N 2 , N 7 ‐trimethylguanosine, inosine, and 1‐methylinosine were each identified in the urine samples from cancer patients. The benefits of the use of LC/MS compared with HPLC alone are discussed. Copyright © 2004 John Wiley & Sons, Ltd.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here

Accelerating Research