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Analysis of urinary nucleosides. IV. Identification of urinary purine nucleosides by liquid chromatography/electrospray mass spectrometry
Author(s) -
Dudley Edward,
Lemière Filip,
Van Dongen Walter,
Tuytten Robin,
ElSharkawi Salah,
Brenton A. Gareth,
Esmans Eddy L.,
Newton Russell P.
Publication year - 2004
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1685
Subject(s) - chemistry , chromatography , mass spectrometry , inosine , urine , electrospray mass spectrometry , high performance liquid chromatography , purine , tandem mass spectrometry , liquid chromatography–mass spectrometry , urinary system , purine metabolism , electrospray , adenosine , biochemistry , enzyme , medicine , endocrinology
Modified urinary nucleosides are potentially invaluable in cancer diagnosis. High‐performance liquid chromatography (HPLC) was combined with full scan mass spectrometry (MS), tandem mass spectrometry and MS n analysis in order to identify purine nucleosides purified from urine. UV peaks evident in the chromatogram were examined by the various mass spectrometric techniques and adenosine, 1‐methyladenosine, xanthosine, N 1 ‐methylguanosine, N 2 ‐methylguanosine, N 2 , N 2 ‐dimethylguanosine, N 2 , N 2 , N 7 ‐trimethylguanosine, inosine, and 1‐methylinosine were each identified in the urine samples from cancer patients. The benefits of the use of LC/MS compared with HPLC alone are discussed. Copyright © 2004 John Wiley & Sons, Ltd.