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Sequence‐ and site‐specific photodissociation at 266 nm of protonated synthetic polypeptides containing a tryptophanyl residue
Author(s) -
Oh Joo Yeon,
Moon Jeong Hee,
Kim Myung Soo
Publication year - 2004
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1679
Subject(s) - chemistry , photodissociation , protonation , peptide , mass spectrometry , tandem , tandem mass spectrometry , chromophore , ion , residue (chemistry) , matrix assisted laser desorption/ionization , photochemistry , fragmentation (computing) , desorption , chromatography , organic chemistry , biochemistry , materials science , composite material , adsorption , computer science , operating system
Abstract Photodissociation at 266 nm of protonated synthetic polypeptides containing a tryptophanyl residue was investigated using a homebuilt tandem time‐of‐flight mass spectrometer equipped with a matrix‐assisted laser desorption/ionization source. Efficient photodissociation of the protonated peptides was demonstrated. Most of the intense peaks in the laser‐induced tandem mass spectra were sequence ions. Furthermore, sequence ions due to cleavages at all the peptide bonds were observed; this is a feature of the technique that is particularly useful for peptide sequencing. Fragmentations at both ends of the tryptophanyl residue were especially prevalent, which can be useful for location of the tryptophanyl chromophore in a peptide. Copyright © 2004 John Wiley & Sons, Ltd.