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High‐resolution analysis by nano‐electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry for the identification of molecular species of phospholipids and their oxidized metabolites
Author(s) -
Ishida Mayuko,
Yamazaki Toshiyuki,
Houjou Toshiaki,
Imagawa Masayoshi,
Harada Ayako,
Inoue Keizou,
Taguchi Ryo
Publication year - 2004
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1650
Subject(s) - chemistry , phosphatidylethanolamine , fourier transform ion cyclotron resonance , phosphatidylcholine , electrospray ionization , mass spectrometry , analytical chemistry (journal) , chromatography , phospholipid , biochemistry , membrane
Nano‐electrospray ionization (ESI) Fourier transform ion cyclotron resonance mass spectrometry (FTICRMS) was applied to identify the molecular species of phosphatidylethanolamine of Caenorhabditis elegans , which has a high concentration of phospholipids with a fatty acyl chain having an odd number of carbon atoms. The molecular species of diacyl phosphatidylethanolamine with one fatty acyl chain having an odd number of carbon atoms and one fatty acyl chain having an even number of carbon atoms was identified separately from alkyl‐acyl phosphatidylethanolamine with an alkyl chain having an even number of carbon atoms and a fatty acyl chain having an even number of carbon atoms. Furthermore, nano‐ESI‐FTICRMS was applied to the direct identification of oxidized phosphatidylcholine from soybean. The mass peaks of individual molecular species of oxidative phosphatidylcholine, such as 34:3 diacyl phosphatidylcholine with peroxide (+2O) ( m/z 788.544) or 34:2 diacyl phosphatidylcholine with peroxide (+2O) ( m/z 790.560), can be separated from the peaks of the molecular species of the non‐oxidative phospholipids. This suggests that the mass peaks with a difference of less than 0.1 mass units in their molecular weight can be separated and that their individual exact molecular compositions can be obtained by the FTICRMS analysis. The high resolution and high accuracy of FTICRMS are very effective in the analysis of molecular species of phospholipids and their derivatives. Copyright © 2004 John Wiley & Sons, Ltd.