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Atmospheric pressure chemical ionization mass spectrometry of aldehydes in biological matrices
Author(s) -
Nagy Kornél,
Pollreisz Ferenc,
Takáts Zoltán,
Vékey Károly
Publication year - 2004
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1648
Subject(s) - chemistry , chromatography , propionaldehyde , atmospheric pressure chemical ionization , derivatization , mass spectrometry , chemical ionization , high performance liquid chromatography , extraction (chemistry) , sample preparation , tandem mass spectrometry , hexane , detection limit , acetone , direct electron ionization liquid chromatography–mass spectrometry interface , analytical chemistry (journal) , ionization , aldehyde , ion , organic chemistry , catalysis
Abstract Application of high‐performance liquid chromatography (HPLC) and tandem mass spectrometry (MS/MS) for detection of aldehydes in biological samples such as blood, yoghurt, and milk, is reported. Sample preparation is easy, and the presented method is both sensitive and selective. It is based on the widely used dinitrophenylhydrazine derivatization, followed by extraction with n‐hexane and a simple reversed‐phase HPLC separation. Detection is performed by atmospheric pressure chemical ionization (APCI) in negative ion mode, with detection limits in the low picogram range. Using MS/MS, acetone and propionaldehyde can clearly be distinguished, facilitating propionaldehyde quantitation even in the presence of high acetone levels. Quantitation by direct MS/MS is also feasible, well suited for high‐throughput applications, although not as accurate as using HPLC/MS/MS. Copyright © 2004 John Wiley & Sons, Ltd.