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The effect of the presence of foreign salts on the formation of gaseous ions for electrospray and laser spray
Author(s) -
Hiraoka Kenzo,
Asakawa Yoshiki,
Kawashima Yoko,
Okazaki Shigemitsu,
Nakamura Masana,
Yamamoto Yasuteru,
Takamizawa Atsushi
Publication year - 2004
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1644
Subject(s) - chemistry , lysozyme , ion , aqueous solution , salt (chemistry) , bromide , chloride , electrospray , inorganic chemistry , chromatography , organic chemistry , biochemistry
The effect of the presence of foreign salts (NaCl, aerosol OT, tetra ‐ n ‐hexylammonium bromide, and CH 3 COONH 4 ) on the formation of gaseous ions for electrospray (ES) and laser spray (LS) was studied in the positive and negative modes of operations. The ion signals for amino acids show sudden decrease with the concentration of foreign salts greater than 10 −5 M for both ES and LS. When the surface‐active counter ions were added, the signal intensities showed a marked decrease for both ES and LS. This may be due to the enrichment of the surface‐active counter ions on the surface of the charged droplets. When CH 3 COONH 4 was added to an aqueous solution of 10 −6 M lysozyme chloride, an increase of the signal intensities for (lysozyme+nH) n+ and a decrease in the values of n were observed. The decrease in n may be due to the salt formation of (lysozyme+nH) n+ with the negative acetate ion leading to the reduction of positive charges. Copyright © 2004 John Wiley & Sons, Ltd.