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Ultra‐performance liquid chromatography coupled to quadrupole‐orthogonal time‐of‐flight mass spectrometry
Author(s) -
Plumb Robert,
CastroPerez Jose,
Granger Jennifer,
Beattie Iain,
Joncour Karine,
Wright Andrew
Publication year - 2004
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1627
Subject(s) - chemistry , chromatography , mass spectrometry , high performance liquid chromatography , analytical chemistry (journal) , resolution (logic) , quadrupole time of flight , particle size , time of flight mass spectrometry , quadrupole , instrumentation (computer programming) , tandem mass spectrometry , ionization , physics , atomic physics , artificial intelligence , computer science , operating system , ion , organic chemistry
Ultra‐performance liquid chromatography (UPLC) utilizes sub‐2 μm particles with high linear solvent velocities to effect dramatic increases in resolution, sensitivity and speed of analysis. The reduction in particle size to below 2 μm requires instrumentation that can operate at pressures in the 6000–15 000 psi range. The typical peak widths generated by the UPLC system are in the order of 1–2 s for a 10‐min separation. In the present work this technology has been applied to the study of in vivo drug metabolism, in particular the analysis of drug metabolites in bile. The reduction in peak width significantly increases analytical sensitivity by three‐ to five‐fold, and the reduction in peak width, and concomitant increase in peak capacity, significantly reduces spectral overlap resulting in superior spectral quality in both MS and MS/MS modes. The application of UPLC/MS resulted in the detection of additional drug metabolites, superior separation and improved spectral quality. Copyright © 2004 John Wiley & Sons, Ltd.