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Matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry as a tool for fast identification of protein binders in color layers of paintings
Author(s) -
Hynek Radovan,
Kuckova Stepanka,
Hradilova Janka,
Kodicek Milan
Publication year - 2004
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1570
Subject(s) - chemistry , mass spectrometry , desorption , matrix (chemical analysis) , ionization , matrix assisted laser desorption/ionization , chromatography , analytical chemistry (journal) , time of flight mass spectrometry , identification (biology) , organic chemistry , adsorption , ion , botany , biology
Identification of materials in color layers of paintings is necessary for correct decisions concerning restoration procedures as well as proving the authenticity of the painting. The proteins are usually important components of the painting layers. In this paper it has been demonstrated that matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOFMS) can be used for fast and reliable identification of proteins in color layers even in old, highly aged matrices. The digestion can be easily performed directly on silica wafers which are routinely used for infrared analysis. The amount of material necessary for such an analysis is extremely small. Peptide mass mapping using digestion with trypsin followed by MALDI‐TOFMS and identification of the protein was successfully used for determination of the binder from a painting of the 19th century. Copyright © 2004 John Wiley & Sons, Ltd.