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Determination of N ‐acetyl‐ S ‐( N ‐methylcarbamoyl)cysteine (AMCC) in urine by high‐performance liquid chromatography combined with mass spectrometry
Author(s) -
Perico A.,
Baglioni S.,
Bavazzano P.
Publication year - 2004
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1565
Subject(s) - chemistry , chromatography , detection limit , mass spectrometry , solid phase extraction , calibration curve , urine , population , extraction (chemistry) , liquid chromatography–mass spectrometry , analytical chemistry (journal) , relative standard deviation , biochemistry , demography , sociology
Abstract A simple, sensitive and specific method for the quantification of N ‐acetyl‐ S ‐( N ‐methylcarbamoyl)cysteine (AMCC) in urines of the general population and of exposed workers has been developed. Samples were first diluted with phosphate buffer followed by purification by solid‐phase extraction (SPE) with SAX columns prior to analysis by liquid chromatography/mass spectrometry (LC/MS). An external standard was used for the quantification together with selected ion monitoring of m/z 219, [MH] − . The linear calibration curve showed a good correlation (r 2 = 0.98, p < 0.001) between 0.1–110 mg/L of AMCC, and the detection limit was calculated to be 2 μg/L. The within‐day and between‐day precision, calculated for exposed workers and general population AMCC levels in urine samples, were determined as 1.2 and 3.6%, respectively (as relative standard deviation (RSD)). Copyright © 2004 John Wiley & Sons, Ltd.