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Thin‐layer chromatography combined with diode laser desorption/atmospheric pressure chemical ionization mass spectrometry
Author(s) -
Peng Song,
Ahlmann Norman,
Kunze Kerstin,
Nigge Walter,
Edler Michael,
Hoffmann Thorsten,
Franzke Joachim
Publication year - 2004
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1556
Subject(s) - chemistry , mass spectrometry , analytical chemistry (journal) , ambient ionization , atmospheric pressure , atmospheric pressure chemical ionization , analyte , desorption electrospray ionization , matrix assisted laser desorption electrospray ionization , atmospheric pressure laser ionization , desorption , ionization , direct electron ionization liquid chromatography–mass spectrometry interface , chromatography , chemical ionization , electron ionization , organic chemistry , adsorption , ion , oceanography , geology
The desorption of an analyte by a continuous wave diode laser from a porous surface of a thin‐layer plate covered with a graphite suspension is presented. The thermally desorbed analyte molecules are ionized in the gas phase by a corona discharge at atmospheric pressure. Therefore, both essential processes—the desorption and the ionization of analyte molecules, which are often performed in one step—are separated. The target preparation is easy and fast since no additional extraction process is required. The mass spectrometric background signal was mostly limited to the low mass range showing no interference with typical compounds of interest. In this study, the calmative and antihypertensive drug reserpine was chosen as model analyte, which is often used for specification of mass spectrometers. No fragmentation was observed because of efficient collisional cooling under atmospheric pressure. The influence of diode laser power and the composition of the graphite suspension were investigated, and a primary optimization was performed. Copyright © 2004 John Wiley & Sons, Ltd.