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Determination of amphetamine in rat brain by in vivo microdialysis and ion‐pairing liquid chromatography with electrospray tandem mass spectrometry
Author(s) -
Fuh MingRen,
Haung ChiuanHung,
Wu TiYu,
Lin ShiangLing,
Pan Wynn H. T.
Publication year - 2004
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1543
Subject(s) - chemistry , microdialysis , chromatography , electrospray , detection limit , tandem mass spectrometry , mass spectrometry , trifluoroacetic acid , liquid chromatography–mass spectrometry , biochemistry , extracellular
An ion‐pairing liquid chromatography/electrospray tandem mass spectrometry (LC/ES‐MS/MS) method with in vivo microdialysis for the determination of amphetamine in rat brain has been developed. A microdialysis probe was surgically implanted into the striatum of the rat and artificial cerebrospinal fluid (aCSF) was used as the perfusion medium. Samples were collected and then analyzed off‐line by LC/ES‐MS/MS. A reversed‐phase C 18 column was employed for LC separation and MS/MS was utilized for detection. Trifluoroacetic acid (TFA) was added to the mobile phase (acetonitrile/water) as an ion‐pairing reagent. Detection was by ES‐MS/MS directly, and no post‐column addition of organic modifier was needed. Dual linear ranges were determined from 0.1–0.5 μg/mL and 0.005–0.1 μg/mL, respectively. The detection limit, based on a signal‐to‐noise ratio of 3, was 0.001 μg/mL (5 nM). Good precision and accuracy were obtained. The applicability of this newly developed method was demonstrated by continuous monitoring of amphetamine concentrations in rat brain. Amphetamine reached a maximum concentration of 0.086 ± 0.017 μg/mL over 20–40 min after a single 3.0 mg/kg intraperitoneal administration. Copyright © 2004 John Wiley & Sons, Ltd.