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Determination of abamectin in soil samples using high‐performance liquid chromatography with tandem mass spectrometry
Author(s) -
Brewer Bobby N.,
Armbrust Kevin L.,
Mead Keith T.,
Holmes William E.
Publication year - 2004
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1537
Subject(s) - abamectin , chemistry , avermectin , chromatography , tandem mass spectrometry , atmospheric pressure chemical ionization , doramectin , mass spectrometry , high performance liquid chromatography , liquid chromatography–mass spectrometry , extraction (chemistry) , pesticide , ivermectin , environmental chemistry , chemical ionization , ionization , medicine , ion , zoology , organic chemistry , anatomy , agronomy , biology
Abamectin, which is comprised of a mixture of avermectins B 1a and B 1b , is a natural pesticide used as an anti‐parasitic agent in livestock, ornamental, and agricultural crops, which can potentially be transported to aquatic systems. These compounds are highly toxic to both aquatic vertebrates and invertebrates at low concentrations in water. This investigation developed high‐performance liquid chromatography/tandem mass spectrometry (HPLC/MS/MS) techniques to support automated extraction by an accelerated solvent extraction (ASE®) system and chromatographic techniques to measure residues of avermectins in complex soil samples. HPLC along with atmospheric pressure chemical ionization (APCI) MS/MS was used for separation and determination of avermectin isomers in soil samples. Average method recovery for abamectin by UV was 91%, while detection by MS/MS resulted in a 68% recovery for abamectin. Individual method recoveries by MS/MS were 53.6% for avermectin B 1a and 36.8% for avermectin B 1b . The use of tandem technology eliminated matrix interferences and resulted in an approximately eight‐fold increase in sensitivity. Copyright © 2004 John Wiley & Sons, Ltd.