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Determination of alkanolamines in cattails ( Typha latifolia ) utilizing electrospray ionization with selected reaction monitoring and ion‐exchange chromatography
Author(s) -
Peru Kerry M.,
Headley John V.,
Doucette William J.
Publication year - 2004
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1531
Subject(s) - chemistry , chromatography , diethanolamine , detection limit , triethanolamine , electrospray ionization , analyte , ion chromatography , electrospray , mass spectrometry , analytical chemistry (journal) , organic chemistry
Selected reaction monitoring (SRM) with electrospray ionization was used as a specific detection technique for the analysis of alkanolamines in plant tissue extracts. Ion‐exchange chromatography was used as the method of separation. Quantification was based on monitoring the loss of either H 2 O or 2(H 2 O) from the protonated molecule [M+H] + . The method provided increased selectivity for all analytes and better detection limits for three of the six analytes investigated compared with an earlier method using selected ion monitoring with liquid chromatography. Instrumental detection limits ranged from 6–300 pg injected for monoethanolamine (MEA), monoisopropanolamine (MIPA), diethanolamine (DEA), methyldiethanolamine (MDEA), diisopropanolamine (DIPA), and triethanolamine (TEA). Method robustness and selectivity were demonstrated by the determination of DIPA and a known transformation product MIPA in over 35 plant extract samples derived from a laboratory study of plant uptake mechanisms. Copyright © 2004 John Wiley & Sons, Ltd.