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Electron capture dissociation Fourier transform ion cyclotron resonance mass spectrometry in the electron energy range 0–50 eV
Author(s) -
Tsybin Youri O.,
Witt Matthias,
Baykut Gökhan,
Håkansson Per
Publication year - 2004
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1525
Subject(s) - chemistry , fourier transform ion cyclotron resonance , ion , atomic physics , electron , mass spectrometry , penning trap , ion cyclotron resonance , fragmentation (computing) , electron capture dissociation , range (aeronautics) , dissociation (chemistry) , irradiation , electron beam ion trap , electron ionization , ionization , cathode ray , cyclotron , physics , materials science , nuclear physics , organic chemistry , chromatography , computer science , composite material , operating system
Electron capture dissociation (ECD) of polypeptide cations was obtained with pencil and hollow electron beams for both sidekick and gas‐assisted dynamic ion trapping (GADT) using Fourier transform ion cyclotron resonance mass spectrometry (FTICR‐MS) with an electrostatic ion transfer line. Increasing the number of trapped ions by multiple ICR trap loads using GADT improved the ECD sensitivity in comparison with sidekick ion trapping and ECD efficiency in comparison with single ion trap load by GADT. Furthermore, enhanced sensitivity made it possible to observe ECD in a wide range of electron energies (0–50 eV). The degree, rate and fragmentation characteristics of ECD FTICR‐MS were investigated as functions of electron energy, electron irradiation time, electron flux and ion trapping parameters for this broad energy range. The results obtained show that the rate of ECD is higher for more energetic (>1 eV) electrons. Long electron irradiation time with energetic electrons reduces average fragment ion mass and decreases efficiency of formation of c ‐ and z ‐type ions. The obtained dependencies suggest that the average fragment ion mass and the ECD efficiency are functions of the total fluence of the electron beam (electron energy multiplied by irradiation time). The measured electron energy distributions in low‐energy ECD and hot ECD regimes are about 1 eV at full width half maximum in employed experimental configurations. Copyright © 2004 John Wiley & Sons, Ltd.