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Small molecule matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry using a polymer matrix
Author(s) -
Soltzberg Leonard J.,
Patel Pina
Publication year - 2004
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1505
Subject(s) - chemistry , mass spectrometry , matrix (chemical analysis) , ionization , analytical chemistry (journal) , matrix assisted laser desorption/ionization , maldi imaging , mass spectrum , polyatomic ion , desorption , time of flight mass spectrometry , small molecule , molecule , analyte , polymer , ion , sample preparation in mass spectrometry , chromatography , organic chemistry , biochemistry , adsorption
We have employed a light‐absorbing electrically conductive polymer as a matrix to determine the molecular mass of small organic molecules using matrix‐assisted laser desorption/ionization time‐of‐flight (MALDI‐TOF) mass spectrometry. This method, which is in contrast to the usual MALDI strategy for matrix selection in which a small molecule matrix is used with a high molecular mass analyte, addresses the problem of matrix interference which limits the usefulness of MALDI‐TOF for small molecule analysis. Use of negative ion mode offers advantages for this application. Using this approach, we have obtained clean molecular ion mass spectra of small organic molecules in the mass range 100–300 Da. Copyright © 2004 John Wiley & Sons, Ltd.