Using sol–gel/crown ether hybrid materials as desalting substrates for matrix‐assisted laser desorption/ionization analysis of oligonucleotides | Zendy

Weng MaoFeng | Zendy; Chen YuChie | Zendy

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Using sol–gel/crown ether hybrid materials as desalting substrates for matrix‐assisted laser desorption/ionization analysis of oligonucleotides

Author(s) -

Weng MaoFeng,

Chen YuChie

Publication year - 2004

Publication title -

rapid communications in mass spectrometry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.528

H-Index - 136

eISSN - 1097-0231

pISSN - 0951-4198

DOI - 10.1002/rcm.1501

Subject(s) - chemistry , crown ether , oligonucleotide , desorption , chromatography , matrix assisted laser desorption/ionization , ether , detection limit , size exclusion chromatography , hydroxymethyl , matrix (chemical analysis) , analytical chemistry (journal) , ion , organic chemistry , adsorption , dna , biochemistry , enzyme

This study demonstrates the feasibility of using sol–gel/crown ether hybrid materials as sample substrates that reduce the intensity of the signals of sodium ion adducts of oligonucleotides during matrix‐assisted laser desorption/ionization (MALDI) analysis. 2‐Hydroxymethyl[15]crown‐5 and 2‐hydroxymethyl[18]crown‐6 were added as dopants during the sol–gel process to generate desalting substrates for MALDI sample deposition. The results demonstrate that the sol–gel/crown ether hybrid materials effectively suppress the formation of sodiated oligonucleotides during MALDI analysis. The largest detectable molecular size for an oligonucleotide was a 100‐mer, and the detection limit for an oligonucleotide 36‐mer was ca. 20 fmol. Copyright © 2004 John Wiley & Sons, Ltd.

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