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Using sol–gel/crown ether hybrid materials as desalting substrates for matrix‐assisted laser desorption/ionization analysis of oligonucleotides
Author(s) -
Weng MaoFeng,
Chen YuChie
Publication year - 2004
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1501
Subject(s) - chemistry , crown ether , oligonucleotide , desorption , chromatography , matrix assisted laser desorption/ionization , ether , detection limit , size exclusion chromatography , hydroxymethyl , matrix (chemical analysis) , analytical chemistry (journal) , ion , organic chemistry , adsorption , dna , biochemistry , enzyme
This study demonstrates the feasibility of using sol–gel/crown ether hybrid materials as sample substrates that reduce the intensity of the signals of sodium ion adducts of oligonucleotides during matrix‐assisted laser desorption/ionization (MALDI) analysis. 2‐Hydroxymethyl[15]crown‐5 and 2‐hydroxymethyl[18]crown‐6 were added as dopants during the sol–gel process to generate desalting substrates for MALDI sample deposition. The results demonstrate that the sol–gel/crown ether hybrid materials effectively suppress the formation of sodiated oligonucleotides during MALDI analysis. The largest detectable molecular size for an oligonucleotide was a 100‐mer, and the detection limit for an oligonucleotide 36‐mer was ca. 20 fmol. Copyright © 2004 John Wiley & Sons, Ltd.