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Characterisation of natural indigo and shellfish purple by mass spectrometric techniques
Author(s) -
Andreotti Alessia,
Bonaduce Ilaria,
Colombini Maria Perla,
Ribechini Erika
Publication year - 2004
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1464
Subject(s) - chemistry , indigo , silylation , mass spectrometry , chromatography , gas chromatography–mass spectrometry , organic chemistry , art , visual arts , catalysis
Two analytical methods based on mass spectrometry were used in the characterisation of constituents of natural indigo prepared from the leaves of Indigofera tinctoria , and of shellfish purple prepared from the hypobranchial glandular secretions of Murex trunculus , following old recipes. On‐line pyrolysis gas chromatography in the presence of hexamethyldisilazane followed by mass spectrometric analysis (Py‐silylation/GC/MS), and direct exposure mass spectrometry (DE‐MS), were used. Extensive fragmentation of indigoid dyes was obtained by Py‐silylation/GC/MS. The following molecular markers were highlighted, which are useful for identification purposes: 1,2‐dihydro‐3 H ‐indol‐3‐one for indigoid dyes, 1,3‐dihydro‐2 H ‐indol‐2‐one for indirubine, and 6‐bromo‐1,2‐dihydro‐3 H ‐indol‐3‐one for shellfish purple. Using DE‐MS, 6,6′dibromoindigotine, monobromoindigotine and indigotine were identified as the main components, and the presence of tyrindoxyl, one of the dye precursors, was also assessed. Copyright © 2004 John Wiley & Sons, Ltd.