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Quantification of cow milk adulteration in goat milk using high‐performance liquid chromatography with electrospray ionization mass spectrometry
Author(s) -
Chen RenKun,
Chang LiWen,
Chung YaYun,
Lee MingHsung,
Ling YongChien
Publication year - 2004
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1460
Subject(s) - chemistry , chromatography , electrospray ionization , mass spectrometry , liquid chromatography–mass spectrometry , direct electron ionization liquid chromatography–mass spectrometry interface , ionization , chemical ionization , ion , organic chemistry
A method was developed for the quantification of cow milk adulteration in goat milk, based on solvent separation of whey proteins followed by high‐performance liquid chromatography with electrospray ionization mass spectrometry (HPLC/ESI‐MS). The presence of cow milk was determined using β ‐lactoglobulin whey protein as the molecular marker. The adulterants were identified using both retention time and molecular mass derived from multiply charged molecular ions. Standard solutions containing cow and goat milk in different volume ratios were prepared and analyzed. Good linearity covering cow milk content from 5% and above was obtained. The proposed method identifies the adulterants using accurate molecular masses for protein identification and detects the addition of cow milk to goat milk at levels as low as 5%. Copyright © 2004 John Wiley & Sons, Ltd.