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A rapid and sensitive method for simultaneous determination of lamivudine and zidovudine in human serum by on‐line solid‐phase extraction coupled to liquid chromatography/tandem mass spectrometry detection
Author(s) -
Estrela Rita de Cassia E.,
Salvadori Myriam C.,
SuarezKurtz Guilherme
Publication year - 2004
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1446
Subject(s) - chemistry , chromatography , solid phase extraction , zidovudine , detection limit , lamivudine , selected reaction monitoring , electrospray ionization , bioequivalence , liquid chromatography–mass spectrometry , tandem mass spectrometry , mass spectrometry , extraction (chemistry) , pharmacokinetics , human immunodeficiency virus (hiv) , pharmacology , virology , virus , medicine , hepatitis b virus , viral disease , biology
Abstract A method based on solid‐phase extraction (SPE) coupled to high‐performance liquid chromatography (HPLC) with positive ion electrospray ionization tandem mass spectrometry (ESI‐MS/MS) detection was developed for the simultaneous determination of lamivudine (3TC) and zidovudine (AZT) in human serum, using didanosine (ddI) as internal standard. The acquisition was performed in multiple reaction monitoring (MRM) mode, monitoring the transitions m/z 230.0 → 111.8 for 3TC, m/z 268.1 → 126.8 for AZT, and m/z 237.2 → 136.8 for ddI. The limits of detection and quantitation were 3 and 10 ng/mL for 3TC, and 5 and 15 ng/mL for AZT. The method was linear in the studied ranges (10–1500 ng/mL for 3TC and 15–3000 ng/mL for AZT), with r 2 > 0.99 for each drug, and the run time was 4 min. The intra‐assay precisions (%) were in the ranges 1.9–8.7 (3TC) and 2.2–8.9 (AZT), the inter‐assay precisions were in the ranges 2.6–9.0 (3TC) and 4.2–8.1 (AZT), and the intra‐ and inter‐assay accuracies were >97% for both drugs. The absolute recoveries were 95–99% for 3TC (45, 600 and 1200 ng/mL) and 104–112% for AZT (45, 1000 and 2400 ng/mL). The analytical method was applied to a bioequivalence study in which 24 healthy adult volunteers received single oral doses of the reference formulation and two test combined AZT/3TC tablets, in an open, three‐period, balanced, randomized, crossover protocol. Based on the 90% confidence interval of the individual ratios (test formulation/reference formulation) for C max (peak serum concentration) and AUC 0‐inf (extrapolated area under the serum concentration vs. time curve from time zero to infinity), it was concluded that the two test formulations are bioequivalent to the reference formulation with respect to the rate and extent of absorption of both 3TC and AZT. Copyright © 2004 John Wiley & Sons, Ltd.