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Derivatization of amino acids with N,N ‐dimethyl‐2,4‐dinitro‐5‐fluorobenzylamine for liquid chromatography/electrospray ionization mass spectrometry
Author(s) -
Liu Zhongfa,
Minkler Paul E.,
Lin De,
Sayre Lawrence M.
Publication year - 2004
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1443
Subject(s) - chemistry , derivatization , chromatography , electrospray ionization , mass spectrometry , high performance liquid chromatography , electrospray , amino acid , biochemistry
Derivatization, separation and identification of amino acids with a novel compound, N,N ‐dimethyl‐2,4‐dinitro‐5‐fluorobenzylamine (DMDNFB), using high‐performance liquid chromatography/electrospray ionization mass spectrometry (HPLC/ESI‐MS) was demonstrated. Compared to derivatization with 2,4‐dinitrofluorobenzene (DNFB), DMDNFB‐derivatized amino acids and dipeptides exhibit much larger ion current signals in the commonly used ESI positive mode, which was attributed to the introduction of the N,N ‐dimethylaminomethyl protonatable site. Copyright © 2004 John Wiley & Sons, Ltd.