The analysis of thyroxine in human serum by an ‘exact matching’ isotope dilution method with liquid chromatography/tandem mass spectrometry | Zendy

Hopley Chris J. | Zendy; Stokes Peter | Zendy; Webb Kenneth S. | Zendy; Baynham Michael | Zendy

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The analysis of thyroxine in human serum by an ‘exact matching’ isotope dilution method with liquid chromatography/tandem mass spectrometry

Author(s) -

Hopley Chris J.,

Stokes Peter,

Webb Kenneth S.,

Baynham Michael

Publication year - 2004

Publication title -

rapid communications in mass spectrometry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.528

H-Index - 136

eISSN - 1097-0231

pISSN - 0951-4198

DOI - 10.1002/rcm.1441

Subject(s) - chemistry , isotope dilution , chromatography , tandem mass spectrometry , mass spectrometry , detection limit , extraction (chemistry) , liquid chromatography–mass spectrometry , solid phase extraction , tandem , dilution , materials science , physics , composite material , thermodynamics

A method for the analysis of thyroxine in human serum, utilising ‘exact matching’ isotope dilution mass spectrometry (IDMS) in combination with liquid chromatography/tandem mass spectrometry (LC/MS/MS), has been developed with a limit of quantification of 0.5 ng g −1 of thyroxine in human serum. The extraction and clean‐up of thyroxine from serum involves an efficient protein‐precipitation stage followed by a solid‐phase extraction procedure to produce an extract essentially free from interfering compounds. The method is reproducible, with expanded uncertainties of less than 2%, and is relatively fast to perform. Copyright © 2004 John Wiley & Sons, Ltd.

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