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The analysis of thyroxine in human serum by an ‘exact matching’ isotope dilution method with liquid chromatography/tandem mass spectrometry
Author(s) -
Hopley Chris J.,
Stokes Peter,
Webb Kenneth S.,
Baynham Michael
Publication year - 2004
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1441
Subject(s) - chemistry , isotope dilution , chromatography , tandem mass spectrometry , mass spectrometry , detection limit , extraction (chemistry) , liquid chromatography–mass spectrometry , solid phase extraction , tandem , dilution , materials science , physics , composite material , thermodynamics
A method for the analysis of thyroxine in human serum, utilising ‘exact matching’ isotope dilution mass spectrometry (IDMS) in combination with liquid chromatography/tandem mass spectrometry (LC/MS/MS), has been developed with a limit of quantification of 0.5 ng g −1 of thyroxine in human serum. The extraction and clean‐up of thyroxine from serum involves an efficient protein‐precipitation stage followed by a solid‐phase extraction procedure to produce an extract essentially free from interfering compounds. The method is reproducible, with expanded uncertainties of less than 2%, and is relatively fast to perform. Copyright © 2004 John Wiley & Sons, Ltd.