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Enhancing capillary liquid chromatography/tandem mass spectrometry of biogenic amines by pre‐column derivatization with 7‐fluoro‐4‐nitrobenzoxadiazole
Author(s) -
Song Yaru,
Quan Zhe,
Evans Joseph L.,
Byrd Edward A.,
Liu YiMing
Publication year - 2004
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1437
Subject(s) - chemistry , chromatography , derivatization , detection limit , mass spectrometry , tandem mass spectrometry , electrospray ionization , biogenic amine , agmatine , selected ion monitoring , gas chromatography–mass spectrometry , putrescine , organic chemistry , biochemistry , receptor , neurotransmitter , enzyme
This paper describes a capillary liquid chromatography/tandem mass spectrometry (LC/MS/MS) determination of biogenic amines enhanced by pre‐column derivatization with 7‐fluoro‐4‐nitrobenzoxadiazole (NBD‐F). Biogenic amines including tryptamine, N ‐methylsalsolinol, histamine, and agmatine were studied. The biogenic NBD‐amine derivatives could be quantitatively enriched in‐line on 20 × 0.25 mm capillary columns packed in‐house with 5 μm C 8 silica particles. In an electrospray ionization (ESI) source these derivatives were ionized effectively, and collision‐induced dissociation (CID) produced predominant characteristic ions allowing sensitive MS/MS detection. Agmatine, a potential neurotransmitter/modulator, was taken as a reference compound to study the analytical figures of merit of the procedure. The detection limit of agmatine was estimated to be 0.6 ng/mL (signal‐to‐noise (S/N) = 3). A linear calibration curve in the range 15–1000 ng/mL agmatine with an r value of 0.9997 was obtained. Tissue samples of rat brain, stomach, and intestine were analyzed. Minimum sample pre‐treatment was needed. Each analysis was accomplished within ca. 12 min. The concentration of agmatine was found to be 0.246, 3.31, and 0.058 μg/g wet tissue in the brain, stomach, and intestine, respectively. Copyright © 2004 John Wiley & Sons, Ltd.