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Atmospheric pressure matrix‐assisted laser desorption/ionization with analysis by ion mobility time‐of‐flight mass spectrometry
Author(s) -
Steiner Wes E.,
Clowers Brian H.,
English William A.,
Hill Herbert H.
Publication year - 2004
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1419
Subject(s) - chemistry , mass spectrometry , analytical chemistry (journal) , ionization , ion mobility spectrometry , atmospheric pressure , ion , photoionization , ion source , atmospheric pressure chemical ionization , fragmentation (computing) , chemical ionization , chromatography , organic chemistry , oceanography , computer science , geology , operating system
The use of an atmospheric pressure matrix‐assisted laser desorption/ionization (AP‐MALDI) source was employed with an atmospheric pressure ion mobility spectrometer (APIMS) and an orthogonal acceleration reflector time‐of‐flight mass spectrometer (TOFMS) to analyze dipeptide and biogenic amine mixtures from a liquid glycerol 2,5‐dihydroxybenzoic acid (DHB) matrix. Improved sensitivities were obtained by the addition of a localized electrical (corona) discharge in conjunction with the AP‐MALDI source. Enhanced sample ionization efficiency created by this combination provided an overall elevation in signal intensity of ∼1.3 orders in magnitude. Combinations of three dipeptides (Gly‐Lys, Ala‐Lys, and Val‐Lys) and nine biogenic amines (dopamine, serotonin, B ‐phenylethylamine, tyramine, octopamine, histamine, tryptamine, spermidine, and spermine) were resolved in less than 18 ms. In many cases, reduced mobility constants ( K o ) were determined for these analytes for the first time. Ion mobility drift times, flight times, arbitrary signal intensities, and collision‐induced dissociation (CID) fragmentation product signatures are reported for each of the samples. Copyright © 2004 John Wiley & Sons, Ltd.