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Anisole, a new dopant for atmospheric pressure photoionization mass spectrometry of low proton affinity, low ionization energy compounds
Author(s) -
Kauppila Tiina J.,
Kostiainen Risto,
Bruins Andries P.
Publication year - 2004
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1408
Subject(s) - chemistry , photoionization , atmospheric pressure chemical ionization , mass spectrometry , electrospray ionization , ionization , direct electron ionization liquid chromatography–mass spectrometry interface , atmospheric pressure , analytical chemistry (journal) , proton affinity , chromatography , anisole , acetonitrile , chemical ionization , ion , organic chemistry , oceanography , protonation , geology , catalysis
Abstract Atmospheric pressure photoionization (APPI) is a novel method of ionization in liquid chromatography/mass spectrometry (LC/MS). It was originally developed in order to broaden the range of LC/MS ionizable compounds towards less polar compounds that cannot be analyzed by electrospray (ESI) and atmospheric pressure chemical ionization (APCI). Studies done thus far have shown that non‐polar compounds that earlier were not ionizable in LC/MS can indeed be ionized by the use of APPI. However, the best ionization efficiency for low polarity samples has been achieved with low proton affinity (PA) solvents that are not suitable in reversed‐phase LC (RP‐LC). Here it is demonstrated that the signals for analytes with low proton affinities in acetonitrile can be increased 100‐fold by using anisole as the dopant for APPI, which takes the sensitivity to the same level achieved in the analysis of high PA analytes. Copyright © 2004 John Wiley & Sons, Ltd.