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Qualitative analysis of phenolic compounds in apple pomace using liquid chromatography coupled to mass spectrometry in tandem mode
Author(s) -
SánchezRabaneda Ferran,
Jáuregui Olga,
LamuelaRaventós Rosa Maria,
Viladomat Francesc,
Bastida Jaume,
Codina Carles
Publication year - 2004
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1370
Subject(s) - chemistry , chromatography , mass spectrometry , cinnamic acid , tandem mass spectrometry , pomace , benzoic acid , tandem , yield (engineering) , liquid chromatography–mass spectrometry , mass spectrum , organic chemistry , biochemistry , materials science , metallurgy , composite material
The occurrence of phenolic compounds in apple residues resulting from the juice industry was investigated to provide an alternative use for this raw material. For the identification of these compounds, liquid chromatography coupled to ionspray mass spectrometry in tandem mode (LC/MS/MS) with negative ion detection was used. The residues were first extracted and then chromatographed on Sephadex LH‐20 to yield 13 fractions. Positive identification of the compounds was based on their retention times and mass spectra in full scan mode (MS), and in different MS/MS modes (product ion scan, precursor ion scan and neutral loss scan). In this way, 60 compounds, including cinnamic and benzoic acid derivatives and flavonoids, were identified, some of them not previously reported in apple waste. Copyright © 2004 John Wiley & Sons, Ltd.