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New fragmentation mechanisms in matrix‐assisted laser desorption/ionization time‐of‐flight/time‐of‐flight tandem mass spectrometry of carbohydrates
Author(s) -
Spina Emanuela,
Sturiale Luisa,
Romeo Donatella,
Impallomeni Giuseppe,
Garozzo Domenico,
Waidelich Dietmar,
Glueckmann Matthias
Publication year - 2004
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1350
Subject(s) - chemistry , fragmentation (computing) , glycosidic bond , tandem mass spectrometry , mass spectrometry , mass spectrum , time of flight mass spectrometry , ion , fast atom bombardment , ionization , tandem , analytical chemistry (journal) , time of flight , desorption , chromatography , organic chemistry , materials science , adsorption , computer science , composite material , enzyme , operating system
The spectra recorded by matrix‐assisted laser desorption/ionization time‐of‐flight/time‐of‐flight tandem mass spectrometry (MALDI‐TOF/TOF‐MS/MS) of complex carbohydrates from human milk are presented. Besides ions originating from glycosidic cleavages and from sugar ring fragmentations, these spectra show intense peaks that may be assigned to ions produced by three new fragmentation pathways involving a six‐atom rearrangement. These ions, together with the A fragments from sugar ring fragmentations, open the possibility of obtaining a complete mapping of the linkage positions present in the carbohydrates investigated by MALDI‐TOF/TOF. Copyright © 2004 John Wiley & Sons, Ltd.