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Structural assignment of isomeric 2‐aminopyridine‐derivatized oligosaccharides using MS n spectral matching
Author(s) -
Takegawa Yasuhiro,
Ito Shinya,
Yoshioka Shinji,
Deguchi Kisaburo,
Nakagawa Hiroaki,
Monde Kenji,
Nishimura ShinIchiro
Publication year - 2004
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1341
Subject(s) - chemistry , ion , mass spectrometry , dissociation (chemistry) , analytical chemistry (journal) , 2 aminopyridine , chromatography , mass spectrum , collision induced dissociation , ionization , electrospray ionization , spectral line , tandem mass spectrometry , medicinal chemistry , organic chemistry , physics , astronomy
Two isomeric pairs of 2‐aminopyridine (PA)‐derivatized fucosylated and non‐fucosylated oligosaccharides (complex‐type N‐glycans of IgG) were analyzed using liquid chromatography/ion trap mass spectrometry (LC/ITMS) with a sonic spray ionization source and by varying the collision‐induced dissociation voltage. Reproducibility of MS n (n = 2) spectra obtained by LC/ITMS was tested considering both fragment ions ( m/z ) and intensities. A comparison of their MS n spectra and evaluation of similarities (or matching), based on correlation coefficients between MS n spectra, was investigated as a possibility for structural assignment of the isomers. It is shown that such MS n spectral matching is useful and applicable to the structural assignment of relatively large fucosylated and sialylated PA‐oligosaccharides released from IgG based on Bn‐ and Yn‐type fragmentations of the corresponding [M+H+Na] 2+ ions. Copyright © 2004 John Wiley & Sons, Ltd.