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Simultaneous determination of the urinary metabolites of benzene, toluene, xylene and styrene using high‐performance liquid chromatography/hybrid quadrupole time‐of‐flight mass spectrometry
Author(s) -
Marchese Stefano,
Curini Roberta,
Gentili Alessandra,
Perret Daniela,
Rocca Lucia Mainero
Publication year - 2004
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1323
Subject(s) - chemistry , chromatography , toluene , mass spectrometry , styrene , quadrupole time of flight , benzene , ethylbenzene , xylene , tandem mass spectrometry , organic chemistry , copolymer , polymer
A simple and rapid method using reversed‐phase liquid chromatography/tandem mass spectrometry (LC/MS/MS) for the simultaneous determination of the urinary metabolites of benzene, toluene, xylene and styrene in human urine specimens and standard solutions is described. A hybrid quadrupole/time‐of‐flight (QqTOF) mass spectrometer was compared for the determination of metabolite of aromatic solvents in urine samples. The metabolites selected were: trans , trans ‐muconic acid, hippuric acid, o ‐, m ‐ and p ‐methylhippuric acid and phenylglyoxylic acid. The compounds were well separated from each other on narrow‐bore 1‐mm i.d. reversed‐phase LC C‐18 columns. Average recoveries for loading 100 μL of urine samples varied from 88–110% and the quantification limits were less than 30 ng/mL for each analyte (3 ng/mL for trans , trans ‐muconic acid). The qualitative information obtained (mass accuracy, resolution and full‐scan spectra) with the QqTOF mass spectrometer allows a secure identification of analytes in biological matrices. Copyright © 2004 John Wiley & Sons, Ltd.