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Molecular mass measurement of polymerase chain reaction products amplified from human blood dna by electrospray ionization mass spectrometry
Author(s) -
Naito Yasuhiro,
Ishikawa Keiichiro,
Koga Yoshinori,
Tsuneyoshi Toshihiro,
Terunuma Hideya,
Arakawa Ryuichi
Publication year - 1995
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290091504
Subject(s) - chemistry , mass spectrometry , polymerase chain reaction , electrospray ionization , dna , chromatography , microbiology and biotechnology , molecular mass , analytical chemistry (journal) , gene , biochemistry , enzyme , biology
We report here on the first analysis of polymerase chain reaction (PCR) products amplified from a small amount of human blood DNA by electrospray ionization mass spectrometry (ESI‐MS). Adenomatous polyposis coli (APC) gene fragment of about 50 base pairs (bp) with a relative molecular mass ( M r ) of approximately 15 000 u was amplified from human blood DNA by PCR. The accurate molecular mass of the PCR products was determined with an accuracy of ∼0.005% by ESI‐MS. The amount of DNA used was only 100 ng (∼ 50 zmol; the theoretically required amount of blood is therefore less than 1 μL for PCR). The ESI‐MS measurement of the PCR products proved to be a new accurate, sensitive and fast tool for gene diagnosis.